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40 years exprince in the Chromotography

ReproSil SEC

ReproSil SEC – for Size Exclusion
Reprosil SEC phases are designed for the Gel Filtration Chromatography (GFC) of
proteins, peptides and
other org. polymers in aqueous systems. In GFC elution order is a function of molecular weight, the largest
molecules eluting first and the smallest last.
The Silica-based Reprosil SEC packings are available with 3 different pore sizes:

References of ReproSil SEC

SEC
Packing: Pore size: MW of Proteins: MW of Linear Molecules:
- - - (denaturated proteins, peptides, other org. Polymers)
Reprosil 50 SEC 50 A° 8 000 – 30 000 D 500 – 10 000 D
Reprosil 200 SEC 200 A° 10 000 – 500 000 D 2 000 – 70 000 D
Reprosil SEC 4000 450 A° 20 000 – 5 000 000 D 4 000 – 500 000 D
Dr Maisch Column:
ReproSil-Pur 50 SEC, 5µm (300 x 8 mm)
Flow: 0,5 ml/min.
Eluent:
50 mM KH2PO4+100mM KCL, pH6,5

Peptide Sample:

1= MW: 3894 D

2= MW: 1593 D

Reprosil Section

3= MW: 826D

Reprosil 200 SEC, 5 µm (300 x 8 mm)

Flow: 1 ml / min

Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0

Sample :

1 =Thyroglobulin (MW – 670 KD)

2 = Ovalbumin (MW – 44 KD)

3 = Myoglobulin (MW – 17 KD

Scientific Section

4 = Phenylalanine Reprosil SEC 4000, 5 µm

A.) 250 x 4.6 mm, Flow: 1 ml / min

B.) 750 x 4.6 mm, Flow: 0,33 ml / min

Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0


Sample:

1= Thyroglobulin (MW – 670 KD)

2= Ovalbumin (MW – 44 KD)

3= Phenylalanine

Typical features for ReproSil 200 SEC, 5 µm (Mat..No.: R25.sec.)

pH-Stability: 2 – 7.5

Pore size: 200 A°

Pore volume: 1.2 ml/g

Capacity: about 10 – 500 µg, depending on column-dimension and protein

Max. pressure: about 200 bars

Dimension: 300 x 8 mm, Eluent: (for all shown separations) 50 mM K-phosphate-buffer, pH=7 + 150 mM KCL, Flow: 1 ml/min., Max.Pressure: 200 bars

Reprosil Maisch

GPC

Polymeric phases for organic GPC: Typical dimensions: 300 x 8 mm or 600 x 8 mm iD. Other dimensions on request. for non aquous GPCDaltons

ReproGel PS GPC, 5 µm, 50 A°< 1 000rg0.050. ReproGel PS GPC, 5 µm, 100 A°> 3000rg0.100. ReproGel PS GPC, 5 µm, 500 A°> 20 000rg0.500. ReproGel PS GPC, 5 µm, 1000 A°1 000 – 40 000rg0.103. ReproGel PS GPC, 5 µm,10 000 A°4 000 – 500 000rg0.104. ReproGel PS GPC, 5 µm, 100 000 A°10 000 – 2 000 000rg0.105. ReproGel PS GPC, 5 µm, 1 000 000 A°200 000 – 10 000 000rg0.106. ReproGel PS GPC, 5 µm, linear1 000 – 1 000 000rg0. lin. Available particle sizes: 3, 5, 10 µm

Orion ReproSil